Comparison of Fluorescent Techniques Using Two Enzymes Catalysed for Measurement of Atmospheric Peroxides

Atmospheric peroxides, especially hydrogen peroxide (H2O2), are essential oxidants. The concentration is closely related to the extent of OH radicals and O3 cycle in tropospheric atmospheric chemistry. However, only a few studies have investigated their concentrations China because inadequacies measurement techniques or higher costs analytical instruments. Therefore, it design suitable analysis method peroxides with sensitivity, lower detection limit, low cost. In view that, this study optimum conditions two H2O2 techniques: high-performance liquid chromatography (HPLC) fluorescence using two-enzyme catalysis horseradishperoxidase (HRP method) Hemin (Hemin method). Furthermore, these methods were systematically compared terms calibration curve, precision, accuracy, applicability for first time. findings showed that HRP had better detecting methyl hydroperoxide (MHP) than method. Moreover, MHP detection, which requires limits high sensitivity. Besides this, inexpensive more hydroxyl alkyl (C ? 3). (average) 0.60 ± 0.37 ppb 0.081 0.039 ppb, respectively, as determined by Importantly, on sunny days cloudy Beijing September 2016. diurnal variation lowest value morning peaks at 13:00–17:00. contrast, highest after 17:00. Photochemical reactions responsible production MHP. olefins emitted motor vehicles also caused increase during evening rush hour.